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ELF5 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ELF5 Antibody (Center) AP7394C
    Western blot analysis of ELF5 Antibody (Center) (Cat. #AP7394c) in MCF7(lane 1),T47D cell line lysates(lane 2) and mouse lung tissues(lane 3) lysates(35ug/lane). ELF5 (arrow) was detected using the purified Pab.
    detail
  • WB - ELF5 Antibody (Center) AP7394C
    Western blot analysis of ELF5 (arrow) using rabbit polyclonal ELF5 Antibody (Center) (Cat. #AP7394c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF5 gene.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UKW6
Other Accession Q8VDK3, Q58DT0
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 31263 Da
Antigen Region 179-207 aa
Additional Information
Gene ID 2001
Other Names ETS-related transcription factor Elf-5, E74-like factor 5, Epithelium-restricted ESE-1-related Ets factor, Epithelium-specific Ets transcription factor 2, ESE-2, ELF5, ESE2
Target/Specificity This ELF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-207 amino acids from the Central region of human ELF5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsELF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ELF5
Synonyms ESE2
Function Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00237}.
Tissue Location Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression
Citations (0)
citation

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Background

ELF5 is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.

References

Choi,Y.S., Biochem. J. 398 (3), 497-507 (2006)
Tummala,R., J. Cell. Biochem. 97 (4), 766-781 (2006)

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Discontinued
Cat# AP7394C
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