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ELF5 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # SizeAvailability Price  
AP7394C 400 µl
(40 western blots)
In Stock
$ 265.00email & fax$ 251.75online
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AP7394C-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
Add to cart
  • Specification
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ELF5 Antibody (Center) - Product Information

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary AccessionQ9UKW6
Other AccessionQ8VDK3, Q58DT0
ReactivityHuman, Mouse
PredictedBovine
Isotype Rabbit Ig
Calculated MW 31263 Da

ELF5 Antibody (Center) - Additional Information

Gene ID 2001
Other Names
ELF5; ESE2; ETS-related transcription factor Elf-5; E74-like factor 5; Epithelium-restricted ESE-1-related Ets factor; Epithelium-specific Ets transcription factor 2
Target/Specificity
This ELF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-207 amino acids from the Central region of human ELF5.
Dilution
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ELF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

ELF5 Antibody (Center) - Protein Information

Name ELF5
Synonyms ESE2
Function
Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.
Cellular Location
Nucleus (By similarity).
Tissue Location
Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression

ELF5 Antibody (Center) - Related Products

BP7394c: ELF5 Antibody (Center) Blocking Peptide

AT1896a: ELF5 Antibody (monoclonal) (M01)

AF2269a: ELF5 Antibody (C-Term)

ELF5 Antibody (Center) - Research Areas

Application Data

  • WB - ELF5 Antibody (Center) AP7394C

    Western blot analysis of ELF5 Antibody (Center) (Cat. #AP7394c) in MCF7(lane 1),T47D cell line lysates(lane 2) and mouse lung tissues(lane 3) lysates(35ug/lane). ELF5 (arrow) was detected using the purified Pab.

  • WB - ELF5 Antibody (Center) AP7394C

    Western blot analysis of ELF5 (arrow) using rabbit polyclonal ELF5 Antibody (Center) (Cat. #AP7394c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF5 gene.

  • WB - ELF5 Antibody (Center) AP7394C
  • WB - ELF5 Antibody (Center) AP7394C

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BACKGROUND

ELF5 is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.

REFERENCES

Choi,Y.S., Biochem. J. 398 (3), 497-507 (2006)
Tummala,R., J. Cell. Biochem. 97 (4), 766-781 (2006)