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RBM3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RBM3 Antibody (Center) AP7397C
    Anti-RBM3 Antibody (Center) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - RBM3 Antibody (Center) AP7397C
    Western blot analysis of RBM3 antibody (Center) (Cat.# AP7397c) in NIH-3T3 cell line lysates (35ug/lane). RBM3 (arrow) was detected using the purified Pab.
    detail
  • WB - RBM3 Antibody (Center) AP7397C
    Western blot analysis of RBM3 (arrow) using rabbit polyclonal RBM3 Antibody (Center) (Cat. #AP7397c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM3 gene.
    detail
  • IHC-P - RBM3 Antibody (Center) AP7397C
    RBM3 Antibody (Center) (RB18776) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RBM3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - RBM3 Antibody (Center) AP7397C
    RBM3 Antibody (Center)(Cat.#AP7397c) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P98179
Other Accession Q925G0, O89086
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 17170 Da
Antigen Region 55-84 aa
Additional Information
Gene ID 5935
Other Names Putative RNA-binding protein 3, RNA-binding motif protein 3, RNPL, RBM3, RNPL
Target/Specificity This RBM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human RBM3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRBM3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RBM3
Synonyms RNPL
Function Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity).
Cellular Location Nucleus. Cytoplasm. Cell projection, dendrite. Note=Localizes in mRNA granules in dentrites.
Research Areas
Citations (0)
citation

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Background

RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this protein is induced by cold shock and low oxygen tension.

References

Zeng,Y., J. Cell. Biochem. 107 (1), 179-188 (2009)
Sureban,S.M., Oncogene 27 (33), 4544-4556 (2008)

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$ 365.00
$ 140.00
Cat# AP7397C
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