- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Other Accession||P67963, P67962|
|Calculated MW||38915 Da|
|Antigen Region||309-337 aa|
|Other Names||Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1|
|Target/Specificity||This CSNK1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-337 amino acids from the C-terminal region of human CSNK1A1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CSNK1A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Note=Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688)|
Provided below are standard protocols that you may find useful for product applications.
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CK1a can phosphorylate a large number of proteins. This cytoplasmic protein participates in Wnt signaling. It has been demonstrated to phosphorylate CTNNB1 on Ser-45 and to interact with the Axin complex.
Liu, C., et al., Cell 108(6):837-847 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Fish, K.J., et al., J. Biol. Chem. 270(25):14875-14883 (1995).
Tapia, C., et al., FEBS Lett. 349(2):307-312 (1994).
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