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LARS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - LARS Antibody (C-term) AP7413b
    Anti-LARS Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LARS Antibody (C-term) AP7413b
    Western blot analysis of anti-LARS Antibody (C-term) (Cat.#AP7413b) in 293 cell line lysates (35ug/lane). LARS(arrow) was detected using the purified Pab.
  • IHC-P - LARS Antibody (C-term) AP7413b
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with LARS antibody (C-term) (Cat.#AP7413b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P2J5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 134466 Da
Antigen Region 1118-1145 aa
Additional Information
Gene ID 51520
Other Names Leucine--tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
Target/Specificity This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1118-1145 amino acids from the C-terminal region of human LARS.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA1352
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
Cellular Location Cytoplasm.
Citations (0)

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LARS, a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. This enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain.


Lue,S.W.; Biochemistry 46 (15), 4466-4472 (2007)
Ling,C., J. Biol. Chem. 280 (41), 34755-34763 (2005)
Giles,R.E., Somatic Cell Genet. 6 (5), 667-687 (1980)

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$ 295.00
$ 99.00
Cat# AP7413b
(40 western blots)
Availability: In Stock
Bulk Size
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