|Application ||WB, IHC-P, E|
|Calculated MW||134466 Da|
|Antigen Region||1118-1145 aa|
|Other Names||Leucine--tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352|
|Target/Specificity||This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1118-1145 amino acids from the C-terminal region of human LARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.|
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Provided below are standard protocols that you may find useful for product applications.
LARS, a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. This enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain.
Lue,S.W.; Biochemistry 46 (15), 4466-4472 (2007)
Ling,C., J. Biol. Chem. 280 (41), 34755-34763 (2005)
Giles,R.E., Somatic Cell Genet. 6 (5), 667-687 (1980)
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