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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   APOA1 Antibody (N-term)   

APOA1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - APOA1 Antibody (N-term) AP7414a
    Western blot analysis of anti-APOA1 Antibody (N-term) (Cat.#AP7414a) in HepG2 cell line lysates (35ug/lane). APOA1(arrow) was detected using the purified Pab.
  • WB - APOA1 Antibody (N-term) AP7414a
    Western blot analysis of APOA1 (arrow) using rabbit polyclonal APOA1 Antibody (N-term) (Cat.#AP7414a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APOA1 gene.
  • IHC-P - APOA1 Antibody (N-term) AP7414a
    Formalin-fixed and paraffin-embedded human Placenta tissue reacted with APOA1 antibody (N-term) (Cat.#AP7414a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - APOA1 Antibody (N-term) AP7414a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOA1 antibody (N-term) (Cat.#AP7414a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - APOA1 Antibody (N-term) AP7414a
    Formalin-fixed and paraffin-embedded human Placenta tissue reacted with APOA1 antibody (N-term) (Cat.#AP7414a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P02647
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30778 Da
Antigen Region 10-37 aa
Additional Information
Gene ID 335
Other Names Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Proapolipoprotein A-I, ProapoA-I, Truncated apolipoprotein A-I, Apolipoprotein A-I(1-242), APOA1
Target/Specificity This APOA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human APOA1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPOA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOA1
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Cellular Location Secreted.
Tissue Location Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
Research Areas
Citations (0)

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Background

Apolipoprotein A-I, is the major protein component of high density lipoprotein (HDL) in plasma. APOA1 promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the APOA1 gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.

References

Simo,R., Arch. Ophthalmol. 126 (8), 1076-1081 (2008)
Shanker,J., Lipids Health Dis 7, 33 (2008)
Brewer,H.B. Jr., Biochem. Biophys. Res. Commun. 80 (3), 623-630 (1978)

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Cat# AP7414a
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(40 western blots)
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