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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GIT1 Antibody (Y510)   

GIT1 Antibody (Y510)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GIT1 Antibody (Y510) AP7416a
    Western blot analysis of anti-GIT1 Antibody (Y510) (Cat.#AP7416a) in mouse cerebellum tissue lysates (35ug/lane). GIT1 (arrow) was detected using the purified Pab.
  • IHC-P - GIT1 Antibody (Y510) AP7416a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GIT1 Antibody (Y510) (Cat.#AP7416a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9Y2X7
Other Accession Q9Z272, Q68FF6
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 84341 Da
Antigen Region 487-517 aa
Additional Information
Gene ID 28964
Other Names ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1
Target/Specificity This GIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-517 amino acids from human GIT1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGIT1 Antibody (Y510) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GIT1
Function GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).
Cellular Location Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin
Research Areas
Citations ( 0 )

Background

GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia.

References

Sato,H., Biochem. Biophys. Res. Commun. 368 (1), 157-161 (2008)
Jones,N.P., Mol. Cell. Biol. 27 (16), 5790-5805 (2007)
Schmalzigaug,R., Cell. Signal. 19 (8), 1733-1744 (2007)
Stockton,R., Mol. Biol. Cell 18 (6), 2346-2355 (2007)

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$ 295.00
$ 99.00
Cat# AP7416a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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