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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GIT1 (Y554) Antibody (C-term)   

GIT1 (Y554) Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GIT1 (Y554) Antibody (C-term) AP7416d
    All lanes : Anti-Phospho-GIT1-pY554. ctrl at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GIT1 (Y554) Antibody (C-term) AP7416d
    GIT1-Y554 Antibody (C-term)(Cat. #AP7416d) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).
  • WB - GIT1 (Y554) Antibody (C-term) AP7416d
    GIT1-Y554 Antibody (C-term)(Cat. #AP7416d) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GIT1 antibody detected the GIT1 protein (arrow).
  • FC - GIT1 (Y554) Antibody (C-term) AP7416d
    GIT1-Y554 Antibody (C-term) (Cat. #AP7416d) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9Y2X7
Other Accession Q9Z272, Q68FF6, NP_054749.2, NP_054749
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 84341 Da
Antigen Region 533-561 aa
Additional Information
Gene ID 28964
Other Names ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1
Target/Specificity This GIT1-Y554 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-561 amino acids from the C-terminal region of human GIT1-Y554.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGIT1 (Y554) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GIT1
Function GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).
Cellular Location Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin
Research Areas
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Background

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (from SwissProt).

References

Hsu, R.M., et al. Mol. Biol. Cell 21(2):287-301(2010)
Hajdo-Milasinovic, A., et al. J. Cell. Sci. 122 (PT 12), 2127-2136 (2009) :
Morimura, S., et al. Biochem. Biophys. Res. Commun. 382(3):614-619(2009)
Wang, J., et al. Arterioscler. Thromb. Vasc. Biol. 29(2):202-208(2009)
Zhan, L., et al. Cell 135(5):865-878(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Webb, D.J., et al. J. Cell. Sci. 119 (PT 14), 2847-2850 (2006) :
Kim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Manabe, R., et al. J. Cell. Sci. 115 (PT 7), 1497-1510 (2002) :
Kawachi, H., et al. Proc. Natl. Acad. Sci. U.S.A. 98(12):6593-6598(2001)
Zhao, Z.S., et al. Mol. Cell. Biol. 20(17):6354-6363(2000)
Premont, R.T., et al. J. Biol. Chem. 275(29):22373-22380(2000)
Premont, R.T., et al. Proc. Natl. Acad. Sci. U.S.A. 95(24):14082-14087(1998)

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$ 295.00
$ 99.00
Cat# AP7416d
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(40 western blots)
Availability: In Stock
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