|Application ||WB, FC, E|
|Other Accession||Q9Z272, Q68FF6, NP_054749.2, NP_054749|
|Calculated MW||84341 Da|
|Antigen Region||533-561 aa|
|Other Names||ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1|
|Target/Specificity||This GIT1-Y554 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-561 amino acids from the C-terminal region of human GIT1-Y554.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GIT1 (Y554) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).|
|Cellular Location||Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin|
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GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (from SwissProt).
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Webb, D.J., et al. J. Cell. Sci. 119 (PT 14), 2847-2850 (2006) :
Kim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005)
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Manabe, R., et al. J. Cell. Sci. 115 (PT 7), 1497-1510 (2002) :
Kawachi, H., et al. Proc. Natl. Acad. Sci. U.S.A. 98(12):6593-6598(2001)
Zhao, Z.S., et al. Mol. Cell. Biol. 20(17):6354-6363(2000)
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Premont, R.T., et al. Proc. Natl. Acad. Sci. U.S.A. 95(24):14082-14087(1998)
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