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PTRF Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PTRF Antibody (C-term) AP7421B
    Western blot analysis of anti-PTRF Antibody (N-term) Pab (Cat.#AP7421b) in A375 cell line lysates (35ug/lane). PTRF(arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q6NZI2
Other Accession P85125, O54724
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43476 Da
Antigen Region 363-390 aa
Additional Information
Gene ID 284119
Other Names Polymerase I and transcript release factor, Cavin-1, PTRF {ECO:0000312|EMBL:AAH661231}
Target/Specificity This PTRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 363-390 amino acids from the C-terminal region of human PTRF.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTRF Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTRF {ECO:0000312|EMBL:AAH66123.1}
Function Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
Cellular Location Membrane, caveola. Cell membrane Microsome. Endoplasmic reticulum {ECO:0000250|UniProtKB:P85125}. Cytoplasm, cytosol. Mitochondrion Nucleus. Note=Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane. Colocalizes with CAV1 in lipid rafts in adipocytes
Citations (0)

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Background

Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.

References

Aboulaich,N., Biochem. Biophys. Res. Commun. 350 (3), 657-661 (2006)
Aboulaich,N., Biochem. J. 383 (PT 2), 237-248 (2004)
Hasegawa,T., Biochem. J. 347 PT 1, 55-59 (2000)

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$ 295.00
$ 99.00
Cat# AP7421B
Size:
Quantity:
(40 western blots)
Availability: In Stock
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