|Application ||WB, E|
|Other Accession||P85972, P26234, Q64727, P12003|
|Predicted||Rat, Pig, Chicken|
|Calculated MW||123799 Da|
|Antigen Region||1012-1039 aa|
|Other Names||Vinculin, Metavinculin, MV, VCL|
|Target/Specificity||This VINC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1012-1039 amino acids from the C-terminal region of human VINC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Vinculin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Note=Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity) Colocalizes with LIMD1 in the focal adhesions.|
|Tissue Location||Metavinculin is muscle-specific.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
VINC is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia.
Moiseyeva E.P., Weller P.A.J. Biol. Chem. 268:4318-4325(1993)
Sun N., Critchley D.R., Paulin D.Biochem. J. 409:657-667(2008)
Izard T., Evans G., Borgon R.A.Nature 427:171-175(2004)
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