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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GOLPH3 Antibody (N-term)   

GOLPH3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GOLPH3 Antibody (N-term) AP7445a
    Western blot analysis of GOLPH3 antibody (N-term) (Cat.#AP7445a) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab.
  • IHC-P - GOLPH3 Antibody (N-term) AP7445a
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - GOLPH3 Antibody (N-term) AP7445a
    Flow cytometric analysis of hela cells using GOLPH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H4A6
Other Accession Q9ERE4, Q9CRA5
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33811 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 64083
Other Names Golgi phosphoprotein 3, Coat protein GPP34, Mitochondrial DNA absence factor, MIDAS, GOLPH3, GPP34
Target/Specificity This GOLPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GOLPH3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGOLPH3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms GPP34
Function Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.
Cellular Location Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion intermembrane space. Cell membrane. Endosome. Note=Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.
Tissue Location Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers
Research Areas
Citations ( 0 )


The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. This protein is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking.


Ota T., Suzuki Y., Nishikawa T.Nat. Genet. 36:40-45(2004) Bell A.W., Ward M.A.J. Biol. Chem. 276:5152-5165(2001)

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$ 295.00
$ 99.00
Cat# AP7445a
(40 western blots)
Availability: In Stock
Bulk Size
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