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SPRY4 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SPRY4 Antibody (C-term) AP7446B
    Western blot analysis of anti-SPRY4 Antibody (C-term) (Cat.#AP7446b) in Sk-Br-3 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9C004
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 32541 Da
Antigen Region 270-301 aa
Additional Information
Gene ID 81848
Other Names Protein sprouty homolog 4, Spry-4, SPRY4
Target/Specificity This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-301 amino acids from the C-terminal region of human SPRY4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPRY4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPRY4
Function Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893). Probably impairs the formation of GTP-Ras (PubMed:12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898).
Cellular Location Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor) (By similarity). Colocalizes with TESK1 in vesicular spots in the cytoplasm (PubMed:15584898) {ECO:0000250|UniProtKB:Q9WTP2, ECO:0000269|PubMed:15584898}
Research Areas
Citations (0)
citation

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Background

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.

References

Tsai CF, et al. (2008) J Proteome Res
Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7
Rikova K, et al. (2007) Cell 131, 1190-203
Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci U S A 104, 5860-5
Tsumura Y, et al. (2005) Biochem J 387, 627-37

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$ 365.00
$ 140.00
Cat# AP7446B
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