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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SPRY4-Y75 Antibody   

SPRY4-Y75 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPRY4-Y75 Antibody AP7446D
    Western blot analysis of Phospho-SPRY4-Y75.ctrl (Cat. #AP7446d) in K562 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab.
  • IHC-P - SPRY4-Y75 Antibody AP7446D
    SPRY4-Y75 Antibody (Cat. #AP7446d) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRY4-Y75 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - SPRY4-Y75 Antibody AP7446D
    SPRY4-Y75 Antibody (Cat. #AP7446d) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9C004
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32541 Da
Antigen Region 26-56 aa
Additional Information
Gene ID 81848
Other Names Protein sprouty homolog 4, Spry-4, SPRY4
Target/Specificity This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human SPRY4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPRY4-Y75 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPRY4
Function Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.
Cellular Location Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side
Research Areas
Citations (0)

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SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.


Tsai CF, et al. (2008) J Proteome Res
Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7
Rikova K, et al. (2007) Cell 131, 1190-203
Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci U S A 104, 5860-5
Tsumura Y, et al. (2005) Biochem J 387, 627-37

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Cat# AP7446D
(40 western blots)
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