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CYP20A1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CYP20A1 Antibody (C-term) AP7453b
    Western blot analysis of CYP20A1(arrow) using rabbit polyclonal CYP20A1 Antibody (C-term)(Cat.#AP7453b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CYP20A1 gene (Lane 2) (Origene Technologies).
    detail
  • IHC-P - CYP20A1 Antibody (C-term) AP7453b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with CYP20A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CYP20A1 Antibody (C-term) AP7453b
    Flow cytometric analysis of NCI-H292 cells using CYP20A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q6UW02
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52432 Da
Antigen Region 364-392 aa
Additional Information
Gene ID 57404
Other Names Cytochrome P450 20A1, 114--, CYP20A1
Target/Specificity This CYP20A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human CYP20A1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP20A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP20A1
Cellular Location Membrane; Single-pass membrane protein
Research Areas
Citations (0)
citation

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Background

CYP20A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen.

References

Clark H.F., Gurney A.L., Abaya E.Genome Res. 13:2265-2270(2003)

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$ 365.00
$ 140.00
Cat# AP7453b
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