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IDH1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IDH1 Antibody (Center) AP7454c
    All lanes : Anti-IDH1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size : 47kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IF - IDH1 Antibody (Center) AP7454c
    Confocal immunofluorescent analysis of IDH1 Antibody (Center)(Cat#AP7454c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - IDH1 Antibody (Center) AP7454c
    Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)(Cat. #AP7454c). AP7454c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • WB - IDH1 Antibody (Center) AP7454c
    Western blot analysis of IDH1 Antibody (Center) (Cat.#AP7454c) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - IDH1 Antibody (Center) AP7454c
    Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center)(Cat#AP7454c). AP7454c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - IDH1 Antibody (Center) AP7454c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - IDH1 Antibody (Center) AP7454c
    IDH1 Antibody (Center) (Cat. #AP7454c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession O75874
Other Accession P41562, O88844, Q9XSG3, Q6XUZ5
Reactivity Human, Mouse
Predicted Bovine, Rat, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 46659 Da
Antigen Region 116-143 aa
Additional Information
Gene ID 3417
Other Names Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH1, PICD
Target/Specificity This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIDH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDH1
Synonyms PICD
Function Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Cellular Location Cytoplasm, cytosol. Peroxisome
Research Areas
Citations (0)
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Background

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

References

Geisbrecht B.V., Gould S.J.J. Biol. Chem. 274:30527-30533(1999)
Xu X., Zhao J., Xu Z.J. Biol. Chem. 279:33946-33957(2004)
Bleeker F.E., Lamba S.Hum. Mutat. 30:7-11(2009)

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$ 365.00
$ 140.00
Cat# AP7454c
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