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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SERPINA4 Antibody (C-term)   

SERPINA4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SERPINA4 Antibody (C-term) AP7455b
    Western blot analysis of SERPINA4 antibody (C-term) (Cat.#AP7455b) in NCI-H460 cell line lysates (35ug/lane). SERPINA4 (arrow) was detected using the purified Pab.
  • IHC-P - SERPINA4 Antibody (C-term) AP7455b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SERPINA4 Antibody (C-term) AP7455b
    SERPINA4 Antibody (C-term) (Cat. #AP7455b) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P29622
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48542 Da
Antigen Region 291-320 aa
Additional Information
Gene ID 5267
Other Names Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, PI-4, Serpin A4, SERPINA4, KST, PI4
Target/Specificity This SERPINA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the C-terminal region of human SERPINA4.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSERPINA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SERPINA4
Synonyms KST, PI4
Function Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
Cellular Location Secreted.
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas
Citations (0)

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Background

SERPINA4 inhibits human amidolytic and kininogenase activities of tissue kallikrein. The inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

References

Chai K.X., Chen L.-M.J. Biol. Chem. 268:24498-24505(1993)
Chai K.X., Ward D.C.Genomics 23:370-378(1994)
Zhou G.X., Chao L.J. Biol. Chem. 267:25873-25880(1992)

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$ 295.00
$ 99.00
Cat# AP7455b
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Quantity:
(40 western blots)
Availability: In Stock
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