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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EN2 Antibody (C-term-2)   

EN2 Antibody (C-term-2)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EN2 Antibody (C-term-2) AP7457D
    Western blot analysis of EN2 antibody (C-term-2) (Cat.# AP7457d) in NCI-H460 cell line lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.
  • IHC-P - EN2 Antibody (C-term-2) AP7457D
    Formalin-fixed and paraffin-embedded human brain with EN2 Antibody (C-term-2), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - EN2 Antibody (C-term-2) AP7457D
    EN2 Antibody (C-term-2) (Cat. #AP7457d) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19622
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34211 Da
Antigen Region 243-271 aa
Additional Information
Gene ID 2020
Other Names Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2
Target/Specificity This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEN2 Antibody (C-term-2) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EN2
Cellular Location Nucleus.
Research Areas
Citations (0)

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EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.


Poole S.J., Law M.L.Genomics 4:225-231(1989)
Benayed R., Gharani N.Am. J. Hum. Genet. 77:851-868(2005)

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$ 295.00
$ 99.00
Cat# AP7457D
(40 western blots)
Availability: In Stock
Bulk Size
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