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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EHD3 Antibody (N-term)   

EHD3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EHD3 Antibody (N-term) AP7458A
    Anti-EHD3 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - EHD3 Antibody (N-term) AP7458A
    Western blot analysis of EHD3 Antibody (N-term) (Cat. #AP7458a) in mouse lung tissue lysates (35ug/lane).EHD3 (arrow) was detected using the purified Pab.
  • IHC-P - EHD3 Antibody (N-term) AP7458A
    EHD3 Antibody (N-term) (Cat. #AP7458a) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EHD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - EHD3 Antibody (N-term) AP7458A
    EHD3 Antibody (N-term) (Cat. #AP7458a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NZN3
Other Accession Q641Z6, Q9WVK4, Q9H4M9, Q5E9R3
Reactivity Human, Mouse
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60887 Da
Antigen Region 22-48 aa
Additional Information
Gene ID 30845
Other Names EH domain-containing protein 3, PAST homolog 3, EHD3, EHD2, PAST3
Target/Specificity This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human EHD3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEHD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EHD3
Synonyms EHD2, PAST3
Function Plays a role in endocytic transport.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein Recycling endosome membrane; Peripheral membrane protein Cytoplasm
Tissue Location Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta
Research Areas
Citations (0)

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EHD3 plays a role in endocytic transport.


Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
Naslavsky, N., et al. J. Cell. Sci. 122 (PT 3), 389-400 (2009)
Roland, J.T., et al. Mol. Biol. Cell 18(8):2828-2837(2007)

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$ 295.00
$ 99.00
Cat# AP7458A
(40 western blots)
Availability: In Stock
Bulk Size
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