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EHD3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EHD3 Antibody (Center) AP7458c
    Western blot analysis of EHD3 Antibody (Center) (Cat.#AP7458c) in CEM cell line lysates (35ug/lane). EHD3 (arrow) was detected using the purified Pab.
  • IHC-P - EHD3 Antibody (Center) AP7458c
    Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - EHD3 Antibody (Center) AP7458c
    Flow cytometric analysis of 293 cells using EHD3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NZN3
Other Accession Q8R491, Q9QXY6
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60887 Da
Antigen Region 189-216 aa
Additional Information
Gene ID 30845
Other Names EH domain-containing protein 3, PAST homolog 3, EHD3, EHD2, PAST3
Target/Specificity This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the Central region of human EHD3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEHD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EHD3
Synonyms EHD2, PAST3
Function Plays a role in endocytic transport.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein Recycling endosome membrane; Peripheral membrane protein Cytoplasm
Tissue Location Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta
Research Areas
Citations (0)

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EHD3 plays a role in endocytic transport.


Pohl U., Smith J.S.Genomics 63:255-262(2000)
George M., Ying G.BMC Cell Biol. 8:3-3(2007)
Sharma M., Naslavsky N.Traffic 9:995-1018(2008)

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$ 295.00
$ 99.00
Cat# AP7458c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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