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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GPT Antibody (N-term P101)   

GPT Antibody (N-term P101)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GPT Antibody (N-term P101) AP7468a
    Western blot analysis of GPT antibody (N-term P101) (Cat.#AP7468a) in HL60 cell line lysates (35ug/lane). GPT (arrow) was detected using the purified Pab.
  • IHC-P - GPT Antibody (N-term P101) AP7468a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - GPT Antibody (N-term P101) AP7468a
    Flow cytometric analysis of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P24298
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54637 Da
Antigen Region 86-112 aa
Additional Information
Gene ID 2875
Other Names Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1
Target/Specificity This GPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-112 amino acids from the N-terminal region of human GPT.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPT Antibody (N-term P101) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPT
Synonyms AAT1, GPT1
Function Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Cellular Location Cytoplasm.
Tissue Location Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue
Research Areas
Citations (0)

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GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.


Yang R.-Z., Blaileanu G.Genomics 79:445-450(2002)
Ishiguro M., Takio K.Biochemistry 30:10451-10457(1991)

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Cat# AP7468a
(40 western blots)
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