|Application ||WB, E|
|Calculated MW||35032 Da|
|Antigen Region||243-272 aa|
|Other Names||Carbonic anhydrase 4, Carbonate dehydratase IV, Carbonic anhydrase IV, CA-IV, CA4|
|Target/Specificity||This CA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the C-terminal region of human CA4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
|Tissue Location||Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels|
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Provided below are standard protocols that you may find useful for product applications.
CA4 is a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This protein is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, the protein may have a role in inherited renal abnormalities of bicarbonate transport.
Okuyama T., Sato S.Proc. Natl. Acad. Sci. U.S.A. 89:1315-1319(1992) Okuyama T.Genomics 16:678-684(1993) Yang Z., Alvarez B.V.Hum. Mol. Genet. 14:255-265(2005) Okuyama T.Arch. Biochem. Biophys. 320:315-322(1995)
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