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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PCM-1 Antibody (C-term)   

PCM-1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PCM-1 Antibody (C-term) AP7481b
    Western blot analysis of PCM-1 antibody (C-eterm) (Cat.# AP7481b) in K562 cell line lysates (35ug/lane). PCM1 (arrow) was detected using the purified Pab.
  • IHC-P - PCM-1 Antibody (C-term) AP7481b
    Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PCM-1 Antibody (C-term) AP7481b
    Flow cytometric analysis of K562 cells using PCM-1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q15154
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 228533 Da
Antigen Region 1816-1845 aa
Additional Information
Gene ID 5108
Other Names Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1
Target/Specificity This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1816-1845 amino acids from the C-terminal region of human PCM-1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCM-1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCM1
Function Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body. Note=Recruitement to the centrosome requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase Displaced from centriolar satellites and centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling
Tissue Location Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
Research Areas
Citations (0)

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Background

PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.

References

Balczon R., Bao L.J. Cell Biol. 124:783-793(1994)
Corvi R., Berger N.Oncogene 19:4236-4242(2000)
Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002)
Reiter A., Walz C.Cancer Res. 65:2662-2667(2005)
Murati A., Gelsi-Boyer V.Leukemia 19:1692-1696(2005)
Hames R.S., Crookes R.E.Mol. Biol. Cell 16:1711-1724(2005)

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Cat# AP7481b
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(40 western blots)
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