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DDX53 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DDX53 Antibody (C-term) AP7491b
    All lanes : Anti-DDX53 Antibody (C-term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: RL95-2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - DDX53 Antibody (C-term) AP7491b
    Flow cytometric analysis of WiDr cells using DDX53 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q86TM3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 71154 Da
Antigen Region 597-626 aa
Additional Information
Gene ID 168400
Other Names Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, CT26, DEAD box protein 53, DEAD box protein CAGE, DDX53, CAGE
Target/Specificity This DDX53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-626 amino acids from the C-terminal region of human DDX53.
Dilution WB~~1:2000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDDX53 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX53
Synonyms CAGE
Cellular Location Nucleus.
Tissue Location Expressed in testis. Wide expression in various cancer tissues and cancer cell lines.
Research Areas
Citations (0)
citation

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References

Shim,H., Lee,H. Biotechnol. Lett. 28 (24), 2057-2063 (2006)
Cho,B., Lee,H. Biochem. Biophys. Res. Commun. 307 (1), 52-63 (2003)
Cho,B., Lim,Y. Biochem. Biophys. Res. Commun. 292 (3), 715-726 (2002)

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$ 365.00
$ 140.00
Cat# AP7491b
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