|Application ||WB, IHC-P, FC, E|
|Other Accession||O88986, Q0P5L8|
|Calculated MW||45285 Da|
|Antigen Region||155-181 aa|
|Other Names||2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL|
|Target/Specificity||This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GCAT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion. Nucleus. Note=Translocates to the nucleus upon cold and osmotic stress|
|Tissue Location||Strongly expressed in heart, brain, liver and pancreas. Also found in lung.|
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Provided below are standard protocols that you may find useful for product applications.
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
Edgar,A.J., Polak,J.M.Eur. J. Biochem. 267 (6), 1805-1812 (2000)
Tressel,T., Thompson,R., J. Biol. Chem. 261 (35), 16428-16437 (1986)
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