GCAT Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, IHC-P, E |
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Primary Accession | O75600 |
Other Accession | O88986, Q0P5L8 |
Reactivity | Human, Mouse |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 45285 Da |
Antigen Region | 155-181 aa |
Gene ID | 23464 |
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Other Names | 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL |
Target/Specificity | This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GCAT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GCAT (HGNC:4188) |
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Synonyms | KBL |
Function | Pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA. |
Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:Q0P5L8}. Nucleus. Note=Translocates to the nucleus upon cold and osmotic stress. |
Tissue Location | Strongly expressed in heart, brain, liver and pancreas. Also found in lung. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
References
Edgar,A.J., Polak,J.M.Eur. J. Biochem. 267 (6), 1805-1812 (2000)
Tressel,T., Thompson,R., J. Biol. Chem. 261 (35), 16428-16437 (1986)
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