|Application ||WB, E|
|Calculated MW||82681 Da|
|Antigen Region||692-721 aa|
|Other Names||Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, ERK-3, MAP kinase isoform p97, p97-MAPK, MAPK6, ERK3, PRKM6|
|Target/Specificity||This ERK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692~721 amino acids from the C-terminal region of human ERK3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ERK3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5.|
|Tissue Location||Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts|
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Provided below are standard protocols that you may find useful for product applications.
ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Coulombe, P., et al., Mol. Cell. Biol. 23(13):4542-4558 (2003).
Meloche, S., et al., Oncogene 13(7):1575-1579 (1996).
Cheng, M., et al., J. Biol. Chem. 271(15):8951-8958 (1996).
Zhu, A.X., et al., Mol. Cell. Biol. 14(12):8202-8211 (1994).
Boulton, T.G., et al., Cell 65(4):663-675 (1991).
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