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JNK2 (MAPK9) Antibody (C-term K373)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7506b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
JNK2 (MAPK9) Antibody (C-term K373) - Product info | |
| Application | WB, IHC
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| Primary Accession | P45984 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 48139 Da |
JNK2 (MAPK9) Antibody (C-term K373) - Additional info | |
| Gene ID 5601 | |
| Other Names MAPK9; JNK2; PRKM9; SAPK1A; Mitogen-activated protein kinase 9; JNK-55; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2 | |
| Target/Specificity This JNK2 (MAPK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366~396 amino acids from the C-terminal region of human JNK2. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions JNK2 (MAPK9) Antibody (C-term K373) is for research use only and not for use in diagnostic or therapeutic procedures. | |
JNK2 (MAPK9) Antibody (C-term K373) - Protein Information | |
| Name MAPK9 | |
| Synonyms JNK2, PRKM9, SAPK1A | |
| Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2 In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway Participates also in neurite growth in spiral ganglion neurons | |
| Cellular Location Cytoplasm. Nucleus. | |
JNK2 (MAPK9) Antibody (C-term K373) - Related products
AP7228c: JNK2 (MAPK9) Antibody (Center)
AP7506a: JNK2 (MAPK9) Antibody (C-term)
AP7506b: JNK2 (MAPK9) Antibody (C-term K373)
RI13198: MAPK9 predesign siRNA
DC06425: Human MAPK9 cDNA Clone
BP7228c: MAPK9 Antibody (Center) Blocking Peptide
BP7506a: JNK2 Antibody (C-term) Blocking Peptide
BP7506b: JNK2 Antibody (C-term K373) Blocking Peptide
AT2793a: MAPK9 Antibody (monoclonal) (M02)
JNK2 (MAPK9) Antibody (C-term K373) - Application data
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Western blot analysis of anti-JNK2 Pab (Cat. #AP7506b) in Jurkat cell lysate. JNK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with JNK2 antibody (C-term K373), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
JNK2 (MAPK9) Antibody (C-term K373) - Research Areas
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BACKGROUND
JNK2 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. JNK2 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. It is inhibited by dual specificity phosphatases, such as DUSP1. The protein has been shown to bind to at least three scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2 and MAPK8IP3/JIP-3/JSAP1. These proteins also bind other components of the JNK signaling pathway
REFERENCES
Gupta, S., et al., EMBO J. 15(11):2760-2770 (1996). Sluss, H.K., et al., Mol. Cell. Biol. 14(12):8376-8384 (1994). Kallunki, T., et al., Genes Dev. 8(24):2996-3007 (1994). Fleming, Y., et al., Biochem. J. 352 Pt 1, 145-154 (2000).
