- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
CDK1 Antibdoy (Y15)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP7517e | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CDK1 Antibdoy (Y15) - Product info | |
| Application | WB
|
| Primary Accession | P06493 |
| Other Accession | P39951, P11440, P23572, P13863, P48734, P24033, P35567 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Drosophila, Mouse, Rat, Xenopus |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 34095 Da |
CDK1 Antibdoy (Y15) - Additional info | |
| Gene ID 983 | |
| Other Names CDK1; CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase | |
| Target/Specificity This CDK1 Antibdoy (Y15) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y14 of human CDK1. | |
| Dilution WB~~1:50~100WB~~1:1000 | |
| Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CDK1 Antibdoy (Y15) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CDK1 Antibdoy (Y15) - Protein Information | |
| Name CDK1 | |
| Synonyms CDC2, CDC28A, CDKN1, P34CDC2 | |
| Function Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins Required in higher cells for entry into S-phase and mitosis Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl- xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C- mediated dephosphorylation and restoring cell cycle progression In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl- xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration | |
| Cellular Location Nucleus. Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic during the interphase. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin- B1. Accumulates in mitochondria in G2-arrested cells upon DNA- damage | |
| Tissue Location Isoform 2 is found in breast cancer tissues. | |
CDK1 Antibdoy (Y15) - Related products
AP1497a: CDC2 Antibody (N-term)
AP1497b: CDC2 Antibody (C-term)
AP3056a: Phospho-CDC2-T161 Antibody
AP3059a: Phospho-CDK1-S39 Antibody
AP3482a: Phospho-CDK1-T14 Antibody
AP7517b: CDC2 (CDK1) Antibody (C-term)
AP7517c: CDK1 Antibody (Center K200)
BP1497a: CDC2 Antibody (N-term) Blocking Peptide
BP1497b: CDC2 Antibody (C-term) Blocking Peptide
BP3056a: Phospho-CDC2-T161 Blocking Peptide
BP3059a: Phospho-CDK1-S39 Antibody Blocking Peptide
BP3482a: Phospho-CDK1-T14 Antibody Blocking Peptide
BP7517b: CDK1 Antibody (C-term) Blocking Peptide
BP7517c: CDK1 Antibody (Center K200) Blocking Peptide
BP7517d: CDK1 Antibody (T14) Blocking Peptide
BP7517e: CDK1 Antibdoy (Y15) Blocking Peptide
CDK1 Antibdoy (Y15) - Application data
-
Western blot analysis of CDK1(arrow) using rabbit polyclonal CDK1 Antibdoy (Y15) (Cat.#AP7517e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK1 gene (Lane 2) (Origene Technologies).
CDK1 Antibdoy (Y15) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CDK1 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Transcript Variant: This variant (1) encodes the full length isoform.
REFERENCES
Kramer, A., et al., Nat. Cell Biol. 6(9):884-891 (2004). Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004). Litvak, V., et al., Mol. Cell 14(3):319-330 (2004). Shapira, M., et al., Cancer 100(8):1615-1621 (2004). Chow, J.P., et al., J. Biol. Chem. 278(42):40815-40828 (2003).
