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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MAK Antibody (C-term)   

MAK Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MAK Antibody (C-term) AP7542b
    Western blot analysis of MAK (arrow) using MAK Antibody (C-term) (Cat.#AP7542b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAK gene (Lane 2) (Origene Technologies).
  • IHC-P - MAK Antibody (C-term) AP7542b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - MAK Antibody (C-term) AP7542b
    MAK Antibody (T593) (Cat. #AP7542b) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the MAK antibody detected the MAK protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20794
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 70581 Da
Antigen Region 578-608 aa
Additional Information
Gene ID 4117
Other Names Serine/threonine-protein kinase MAK, Male germ cell-associated kinase, MAK
Target/Specificity This MAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 578-608 amino acids from the C-terminal region of human MAK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAK
Function Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment. Note=Localized in both the connecting cilia and the outer segment axonemes (By similarity). Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase.
Tissue Location Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
Research Areas
Citations ( 0 )


MAK is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.


Xia, L., et al., J. Biol. Chem. 277(38):35422-35433 (2002). Taketo, M., et al., Genomics 19(2):397-398 (1994). Jinno, A., et al., Mol. Cell. Biol. 13(7):4146-4156 (1993). Bladt, F., et al., Differentiation 53(2):115-122 (1993). Koji, T., et al., Cell Biochem. Funct. 10(4):273-279 (1992).

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$ 295.00
$ 99.00
Cat# AP7542b
(40 western blots)
Availability: In Stock
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