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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NLK-T286 Antibody (Center)   

NLK-T286 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NLK-T286 Antibody (Center) AP7545c
    NLK-T286 (Cat. #AP7545c) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).
  • FC - NLK-T286 Antibody (Center) AP7545c
    NLK-T286 Antibody (Center) (Cat. #AP7545c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - NLK-T286 Antibody (Center) AP7545c
    Confocal immunofluorescent analysis of NLK-T286 Antibody (Center)(Cat#AP7545c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UBE8
Other Accession D3ZSZ3, O54949, E1BMN8, NP_057315.3
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58283 Da
Antigen Region 275-300 aa
Additional Information
Gene ID 51701
Other Names Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1, NLK, LAK1 {ECO:0000312|EMBL:AAD560131}
Target/Specificity This NLK-T286 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-300 amino acids from the Central region of human NLK-T286.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNLK-T286 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NLK
Synonyms LAK1 {ECO:0000312|EMBL:AAD56013.1}
Function Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1.
Cellular Location Nucleus. Cytoplasm. Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity).
Research Areas
Citations (0)

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Kim, S., et al. J. Biol. Chem. 285(11):8122-8129(2010)
Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
Shi, Y., et al. Mol. Cell. Biochem. 333 (1-2), 293-298 (2010) :
Emami, K.H., et al. Prostate 69(14):1481-1492(2009)
Saijo, K., et al. Cell 137(1):47-59(2009)
Wistow, G., et al. Mol. Vis. 8, 196-204 (2002) :
Kortenjann, M., et al. Gene 278 (1-2), 161-165 (2001) :
Kehrer-Sawatzki, H., et al. Gene 251(1):63-71(2000)
Brott, B.K., et al. Proc. Natl. Acad. Sci. U.S.A. 95(3):963-968(1998)
Volorio, S., et al. DNA Seq. 9 (5-6), 307-315 (1998) :

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$ 295.00
$ 99.00
Cat# AP7545c
(40 western blots)
Availability: In Stock
Bulk Size
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