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MAPK14 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # SizeAvailability Available Date  
AP7546b 400 µl
(40 western blots)
Backorder 4 months
AP7546b-ev 80 µl
(8 western blots)
Backorder 4 months
  • Specification
  • Citations : 2
  • Reviews
  • Protocols
  • Background

MAPK14 Antibody (C-term) - Product info

Application
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IF
Primary AccessionQ16539
Other AccessionP47811
ReactivityHuman
PredictedMouse
IsotypeRabbit Ig
Calculated MW41293 Da

MAPK14 Antibody (C-term) - Additional info

Gene ID 1432
Other Names
MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; Cytokine suppressive anti-inflammatory drug-binding protein; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; Stress-activated protein kinase 2a
Target/Specificity
This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the C-terminal region of human MAPK14.
Dilution
WB~~1:1000
FC~~1:10~50
IF~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
MAPK14 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

MAPK14 Antibody (C-term) - Protein Information

Name MAPK14
Synonyms CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF- induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14- mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF- kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'.
Cellular Location
Cytoplasm. Nucleus.
Tissue Location
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney

MAPK14 Antibody (C-term) - Related products

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AP7226e: MAPK14 Antibody (T179)

AP7226f: MAPK14 Antibody (T180/Y182)

BP12488c: p38 MAPK Antibody (Center T180/Y182) Blocking Peptides

BP16228a: Mouse Mapk14 Antibody (N-term) Blocking Peptide

BP3422a: Phospho-MAPK14-pT179 Antibody Blocking Peptide

BP3438a: Phospho-MAPK14-pY323 Antibody Blocking Peptide

BP3604a: Phospho-MAPK14-pY182 Antibody Blocking Peptide

BP3642a: Phospho-p38 MAPK-pT180 Antibody Blocking Peptide

BP3643a: Biphospho-p38 MAPK-pT180/Y182 Antibody Blocking Peptide

BP7226d: MAPK14 Antibody (Y323) Blocking Peptide

BP7226e: MAPK14 Antibody (T179) Blocking Peptide

BP7226f: MAPK14 Antibody (T180/Y182) Blocking Peptide

BP7508a: P38 Antibody (N-term) Blocking Peptide

BP7546b: P38 Antibody (C-term) Blocking Peptide

AJ1571a: MAPK14 Antibody

AN1020: Phospho-Thr180/Tyr182 p38 MAPK Antibody

AP50174: Phospho-p38 MAPK (Tyr322) Antibody

AP50545: Phospho-p38 MAPK (Tyr182) Antibody

AP50546: Phospho-p38 MAPK (Thr180) Antibody

AO1886a: MAPK14 Antibody

AO1950a: MAPK14 Antibody

MAPK14 Antibody (C-term) - Research Areas

Application data

  • WB - MAPK14 Antibody (C-term) AP7546b

    The anti-P38 Pab (Cat. #AP7546b) is used in Western blot to detect P38 in Jurkat cell lysate.

  • WB - MAPK14 Antibody (C-term) AP7546b

    Western blot analysis of P38 (arrow) using P38 Antibody (C-term) (Cat.#AP7546b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (Lane 2) (Origene Technologies).

  • FC - MAPK14 Antibody (C-term) AP7546b

    MAPK14 Antibody (C-term) (Cat. #AP7546b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • IF - MAPK14 Antibody (C-term) AP7546b

    Confocal immunofluorescent analysis of MAPK14 Antibody (C-term)(Cat#AP7546b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • WB - MAPK14 Antibody (C-term) AP7546b
  • WB - MAPK14 Antibody (C-term) AP7546b
  • FC - MAPK14 Antibody (C-term) AP7546b
  • IF - MAPK14 Antibody (C-term) AP7546b

ELITE™ Custom Antibody Services

  • Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells. Author : Tetsunaga T, Nishida K, Furumatsu T, Naruse K, Hirohata S, Yoshida A, Saito T, Ozaki T.
    Osteoarthritis Cartilage. 2011 Feb;19(2):222-32. doi: 10.1016/j.joca.2010.11.004. Epub 2010 Nov 19.
    PubMed® Id : 21094261
  • Activation of p38 MAPK and increased glucose transport in chronic hibernating swine myocardium. Author : McFalls EO, Hou M, Bache RJ, Best A, Marx D, Sikora J, Ward HB.
    Am J Physiol Heart Circ Physiol. 2004 Sep;287(3):H1328-34.
    PubMed® Id : 15317680

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

P38 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

REFERENCES

Cheung, P.C., et al., EMBO J. 22(21):5793-5805 (2003).
Dean, J.L., et al., J. Biol. Chem. 278(41):39470-39476 (2003).
Sun, A., et al., Exp. Neurol. 183(2):394-405 (2003).
Yustein, J.T., et al., Oncogene 22(40):6129-6141 (2003).
Frevel, M.A., et al., Mol. Cell. Biol. 23(2):425-436 (2003).