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CLIC4 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9Y696 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 28772 Da |
| Gene ID | 25932 |
|---|---|
| Other Names | Chloride intracellular channel protein 4, Intracellular chloride ion channel protein p64H1, CLIC4 |
| Target/Specificity | This CLIC4 antibody is generated from rabbits immunized with recombinant human CLIC4 protein. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CLIC4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLIC4 |
|---|---|
| Function | Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). |
| Cellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane; Single-pass membrane protein. Nucleus. Cell membrane; Single-pass membrane protein. Mitochondrion {ECO:0000250|UniProtKB:Q9Z0W7}. Cell junction. Note=Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles. Might not be present in the nucleus of cardiac cells. {ECO:0000250|UniProtKB:Q9Z0W7, ECO:0000269|PubMed:14569596} |
| Tissue Location | Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle. |
Provided below are standard protocols that you may find useful for product applications.
Background
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
References
Singh,H.,FEBS J. 274 (24), 6306-6316 (2007)
Suh,K.S.,J. Cell. Sci. 120 (PT 15), 2631-2640 (2007)
Suh,K.S.,Clin. Cancer Res. 13 (1), 121-131 (2007)
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