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HARS Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P12081 |
| Other Accession | Q61035, Q2KI84 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57411 Da |
| Antigen Region | 49-78 aa |
| Gene ID | 3035 |
|---|---|
| Other Names | Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS, HARS, HRS |
| Target/Specificity | This HARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human HARS. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HARS1 (HGNC:4816) |
|---|---|
| Synonyms | HARS, HRS |
| Function | Catalyzes the ATP-dependent ligation of histidine to the 3'- end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed:29235198). Plays a role in axon guidance (PubMed:26072516). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:F1Q5D5}. |
| Tissue Location | Brain, heart, liver and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The protein is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
References
Levine,S.M., Arthritis Rheum. 56 (8), 2729-2739 (2007)
Lu,Q., Proc. Natl. Acad. Sci. U.S.A. 100 (13), 7626-7631 (2003)
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