|Application ||WB, IHC-P, E|
|Other Accession||Q61035, Q2KI84|
|Calculated MW||57411 Da|
|Antigen Region||49-78 aa|
|Other Names||Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS, HARS, HRS|
|Target/Specificity||This HARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human HARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytoplasmic histidine--tRNA ligase (Probable). Plays a role in axon guidance.|
|Tissue Location||Brain, heart, liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The protein is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Levine,S.M., Arthritis Rheum. 56 (8), 2729-2739 (2007)
Lu,Q., Proc. Natl. Acad. Sci. U.S.A. 100 (13), 7626-7631 (2003)
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