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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PUM1 Antibody (Y83)   

PUM1 Antibody (Y83)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PUM1 Antibody (Y83) AP7569d
    Anti-PUM1 Antibody (Y83) at 1:1000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 126 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PUM1 Antibody (Y83) AP7569d
    Western blot analysis of PUM1 (arrow) using PUM1 Antibody (Y83) (Cat.#AP7569d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM1 gene (Lane 2) (Origene Technologies).
  • IHC-P - PUM1 Antibody (Y83) AP7569d
    Formalin-fixed and paraffin-embedded humanbrain tissue reacted with Phospho-PUM1-Y83.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14671
Other Accession Q80U78, Q2VB19
Reactivity Human
Predicted Mouse, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 126473 Da
Antigen Region 62-91 aa
Additional Information
Gene ID 9698
Other Names Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, KIAA0099, PUMH1
Target/Specificity This PUM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from human PUM1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPUM1 Antibody (Y83) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PUM1
Synonyms KIAA0099, PUMH1
Function Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. May be required to support proliferation and self-renewal of stem cells.
Cellular Location Cytoplasm.
Tissue Location Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested
Research Areas
Citations (0)

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PUM1 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.


Gupta,Y.K., Structure 16 (4), 549-557 (2008)
Spassov,D.S.,IUBMB Life 55 (7), 359-366 (2003)
Spassov,D.S.,Gene 299 (1-2), 195-204 (2002)

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$ 295.00
$ 99.00
Cat# AP7569d
(40 western blots)
Availability: In Stock
Bulk Size
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