|Application ||WB, IHC-P, E|
|Other Accession||Q80U78, Q2VB19|
|Calculated MW||126473 Da|
|Antigen Region||62-91 aa|
|Other Names||Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, KIAA0099, PUMH1|
|Target/Specificity||This PUM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from human PUM1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PUM1 Antibody (Y83) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. May be required to support proliferation and self-renewal of stem cells.|
|Tissue Location||Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested|
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Provided below are standard protocols that you may find useful for product applications.
PUM1 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.
Gupta,Y.K., Structure 16 (4), 549-557 (2008)
Spassov,D.S.,IUBMB Life 55 (7), 359-366 (2003)
Spassov,D.S.,Gene 299 (1-2), 195-204 (2002)
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