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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EARS2 Antibody (C-term)   

EARS2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EARS2 Antibody (C-term) AP7573b
    Western blot analysis of anti-EARS2 Pab (Cat.#AP7573b) in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab.
  • IHC-P - EARS2 Antibody (C-term) AP7573b
    Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) (Cat.#AP7573b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5JPH6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58689 Da
Antigen Region 495-523 aa
Additional Information
Gene ID 124454
Other Names Probable glutamate--tRNA ligase, mitochondrial, Glutamyl-tRNA synthetase, GluRS, EARS2, KIAA1970
Target/Specificity This EARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-523 amino acids from the C-terminal region of human EARS2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEARS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EARS2
Synonyms KIAA1970
Function Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
Cellular Location Mitochondrion matrix.
Research Areas
Citations ( 0 )


Glutamyl-tRNA synthetase (GluRS or EARS2) a class I aminoacyl-tRNA synthetase (aaRS), is primarily responsible for the glutamylation of tRNAGlu. It is part of the ?minimal set?of seventeen aaRSs found in every living organism and its presence is essential for the viability of cells.


Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)

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$ 295.00
$ 99.00
Cat# AP7573b
(40 western blots)
Availability: In Stock
Bulk Size
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