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Cullin 4A (CUL4A) Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP7590b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Cullin 4A (CUL4A) Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | Q13619 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 87680 Da |
Cullin 4A (CUL4A) Antibody (C-term) - Additional info | |
| Gene ID 8451 | |
| Other Names CUL4A; Cullin-4A | |
| Target/Specificity This Cullin 4A (CUL4A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 630~660 amino acids from the C-terminal region of human CUL4a. | |
| Dilution WB~~1:50~100WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Cullin 4A (CUL4A) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Cullin 4A (CUL4A) Antibody (C-term) - Protein Information | |
| Name CUL4A | |
| Function Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9 | |
Cullin 4A (CUL4A) Antibody (C-term) - Related products
AP7590a: Cullin 4A (CUL4A) Antibody (N-term)
AP7590b: Cullin 4A (CUL4A) Antibody (C-term)
RI11324: CUL4A predesign siRNA
Cullin 4A (CUL4A) Antibody (C-term) - Application data
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Western blot analysis of CUL4a (arrow) using rabbit polyclonal CUL4a Antibody (Human C-term) (Cat.#AP7590b).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CUL4a gene (Lane 2) (Origene Technologies).
Cullin 4A (CUL4A) Antibody (C-term) - Research Areas
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BACKGROUND
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitiantion of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
REFERENCES
Huang,J.,Oncogene 27 (29), 4056-4064 (2008) Hu,J.,Genes Dev. 22 (7), 866-871 (2008) Wen,X., J. Biol. Chem. 282 (37), 27046-27057 (2007)
