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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EphB3 Antibody   

EphB3 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EphB3 Antibody AP7624d
    Western blot analysis of EphB3 Antibody (Cat. #AP7624d) in C6 cell line lysates (35ug/lane).EphB3 (arrow) was detected using the purified Pab
  • WB - EphB3 Antibody AP7624d
    Western blot analysis of EphB3 Antibody (Cat. #AP7624d) in mouse stomach tissue lysates (35ug/lane).EphB3 (arrow) was detected using the purified Pab.
  • FC - EphB3 Antibody AP7624d
    EphB3 Antibody (Cat. #AP7624d) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54753
Other Accession NP_004434
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 110330 Da
Additional Information
Gene ID 2049
Other Names Ephrin type-B receptor 3, EPH-like tyrosine kinase 2, EPH-like kinase 2, Embryonic kinase 2, EK2, hEK2, Tyrosine-protein kinase TYRO6, EPHB3, ETK2, HEK2, TYRO6
Target/Specificity This EphB3 antibody is generated from rabbits immunized with EphB3 his fusion protein.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEphB3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPHB3
Synonyms ETK2, HEK2, TYRO6
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Cellular Location Cell membrane; Single-pass type I membrane protein Cell projection, dendrite
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB3, a member of the Tyr family of protein kinases, is a receptor for members of the ephrin-B family; it binds to both ephrin-B1 and -B2. Expression of this Type I membrane protein is ubiquitous. The protein contains putative domains for 2 fibronectin type III and 1 sterile alpha motif (SAM).


Chiu,S.T.,, Carcinogenesis 30 (9), 1475-1486 (2009)
Kang,J.U.,, BMC Cancer 9, 237 (2009)

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$ 295.00
$ 99.00
Cat# AP7624d
(40 western blots)
Availability: In Stock
Bulk Size
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