- CITATIONS: 3
|Application ||WB, IHC-P, E|
|Calculated MW||110700 Da|
|Antigen Region||990-1021 aa|
|Other Names||Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6|
|Target/Specificity||This EphB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 990-1021 amino acids from the C-terminal region of human EphB6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EphB6 Antibody (C-term H990) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues.|
Provided below are standard protocols that you may find useful for product applications.
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.
Freywald, A., et al., J. Biol. Chem. 278(12):10150-10156 (2003). Luo, H., et al., J. Clin. Invest. 110(8):1141-1150 (2002). Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). Luo, H., et al., J. Immunol. 167(3):1362-1370 (2001). Tang, X.X., et al., Proc. Natl. Acad. Sci. U.S.A. 97(20):10936-10941 (2000).
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