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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EGFR Antibody (S1070)   

EGFR Antibody (S1070)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EGFR Antibody (S1070) AP7628s
    Western blot analysis of anti-EGFR Antibody (S1070) (Cat.#AP7628s) in kidney heart tissue lysates (35ug/lane). EGFR(arrow) was detected using the purified Pab.
  • WB - EGFR Antibody (S1070) AP7628s
    Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).
  • IHC-P - EGFR Antibody (S1070) AP7628s
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EGFR Antibody (S1070), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P00533
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 134277 Da
Antigen Region 1048-1077 aa
Additional Information
Gene ID 1956
Other Names Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Target/Specificity This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from human EGFR.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGFR Antibody (S1070) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EGFR
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Research Areas
Citations (0)

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Background

The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

References

Zanardi, T.A., et al., J. Virol. 77(21):11685-11696 (2003).
Krug, A.W., et al., J. Biol. Chem. 278(44):43060-43066 (2003).
Huang, F., et al., J. Biol. Chem. 278(44):43411-43417 (2003).
He, Y.Y., et al., J. Biol. Chem. 278(43):42457-42465 (2003).
Hirsch, F.R., et al., J. Clin. Oncol. 21(20):3798-3807 (2003).

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$ 295.00
$ 99.00
Cat# AP7628s
Size:
Quantity:
(40 western blots)
Availability: In Stock
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