NAB2 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q15742 |
Other Accession | Q61127 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 56594 Da |
Antigen Region | 109-138 aa |
Gene ID | 4665 |
---|---|
Other Names | NGFI-A-binding protein 2, EGR-1-binding protein 2, Melanoma-associated delayed early response protein, Protein MADER, NAB2, MADER |
Target/Specificity | This NAB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human NAB2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NAB2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NAB2 |
---|---|
Synonyms | MADER |
Function | Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity). |
Cellular Location | Nucleus. Note=Isoform 2 is not localized to the nucleus. |
Tissue Location | Widely expressed at low levels. Highly expressed in melanoma cell lines |
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Provided below are standard protocols that you may find useful for product applications.
Background
NAB2 is a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by this protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex.
References
Srinivasan,R., J. Biol. Chem. 281 (22), 15129-15137 (2006)
Kumbrink,J., J. Biol. Chem. 280 (52), 42785-42793 (2005)
Lucerna,M., J. Biol. Chem. 278 (13), 11433-11440 (2003)
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