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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ILK1/ILK2 Antibody (N-term)   

ILK1/ILK2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ILK1/ILK2 Antibody (N-term) AP7651a
    Western blot analysis of anti-ILK1 Pab (Cat. #AP7651a) in HL60 cell lysate. ILK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - ILK1/ILK2 Antibody (N-term) AP7651a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - ILK1/ILK2 Antibody (N-term) AP7651a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK1/ILK2 Antibody (N-term) (Cat.#AP7651a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O55222
Other Accession Q99J82, Q13418, Q3SWY2
Reactivity Human
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51373 Da
Antigen Region 11-41 aa
Additional Information
Gene ID 16202
Other Names Integrin-linked protein kinase, Ilk
Target/Specificity This ILK1/ILK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of mouse ILK1/ILK2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsILK1/ILK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Ilk
Function Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side {ECO:0000250|UniProtKB:Q13418}. Cytoplasm, myofibril, sarcomere {ECO:0000250|UniProtKB:Q13418}. Cell projection, lamellipodium
Tissue Location Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis
Research Areas
Citations ( 0 )

Background

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK encodes a predicted 451-amino acid protein, with an apparent molecular weight of 59 kD. The ILK protein is a serine/threonine protein kinase with 4 ankyrin-like repeats. ILK regulates integrin-mediated signal transduction.

References

Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003).
Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003).
Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003).
Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003).
Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).

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$ 295.00
$ 99.00
Cat# AP7651a
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Quantity:
(40 western blots)
Availability: In Stock
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