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c-KIT Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7656a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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c-KIT Antibody (N-term) - Product info | |
| Application | WB, IHC, FC
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| Primary Accession | P10721 |
| Other Accession | Q99662 |
| Reactivity | Human, Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 109865 Da |
c-KIT Antibody (N-term) - Additional info | |
| Gene ID 3815 | |
| Other Names KIT; SCFR; Mast/stem cell growth factor receptor Kit; Piebald trait protein; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD_antigen=CD117; Flags: Precursor | |
| Target/Specificity This c-KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human KIT. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions c-KIT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
c-KIT Antibody (N-term) - Protein Information | |
| Name KIT | |
| Synonyms SCFR | |
| Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1 | |
| Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head | |
| Tissue Location Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells | |
c-KIT Antibody (N-term) - Related products
AP7656a: c-KIT Antibody (N-term)
AP7656b: c-KIT Antibody (C-term)
AP7656e: KIT Antibody (N-term D121)
AP3538a: Phospho-KIT-Y721 Antibody
AP3569a: Phospho-KIT-pS741 Antibody
AP3570a: Phospho-KIT-pY703 Antibody
AP7656h: KIT Antibody (C-term)
BP3538a: Phospho-KIT-Y721 Antibody Blocking Peptide
BP3569a: Phospho-KIT-pS741 Antibody Blocking Peptide
BP3570a: Phospho-KIT-pY703 Antibody Blocking Peptide
BP7656a: KIT Antibody (N-term) Blocking Peptide
BP7656b: KIT Antibody (C-term) Blocking Peptide
BP7656f: KIT Antibody (Y721) Blocking Peptide
BP7656g: KIT Antibody (Y703) Blocking Peptide
BP7656h: KIT Antibody (C-term) Blocking Peptide
AJ1189a: c-Kit/CD117 Antibody (C-term)
AE1008a: C-Kit Antibody (Y721)
AE1008b: C-Kit Antibody (Y936)
c-KIT Antibody (N-term) - Application data
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The anti-KIT Pab (Cat. #AP7656a) is used in Western blot to detect KIT in serum-starved HeLa cell lysate (lane 1) and primate testis tissue lysate (lane 2).
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Purified rabbit anti-KIT Pab was used in immunohistochemistry on Breast Carcinoma.
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Flow cytometric analysis of hela cells using KIT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
c-KIT Antibody (N-term) - Research Areas
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BACKGROUND
KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
REFERENCES
Wardelmann, E., et al., Int. J. Cancer 106(6):887-895 (2003). Lennartsson, J., et al., Exp. Cell Res. 288(1):110-118 (2003). Sakuma, Y., et al., Cancer Sci 94(6):486-491 (2003). Araki, K., et al., Lung Cancer 40(2):173-180 (2003). Voytyuk, O., et al., J. Biol. Chem. 278(11):9159-9166 (2003).
