|Application ||WB, E|
|Other Accession||Q4R4M3, P04836|
|Calculated MW||53151 Da|
|Antigen Region||381-412 aa|
|Other Names||Carboxypeptidase E, CPE, Carboxypeptidase H, CPH, Enkephalin convertase, Prohormone-processing carboxypeptidase, CPE|
|Target/Specificity||This CPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-412 amino acids from the C-terminal region of human CPE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CPE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.|
|Cellular Location||Isoform 1: Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted. Note=Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions.|
|Tissue Location||Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
CPE is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in CPE gene are implicated in type II diabetes.
Oiso,S., J. Neurochem. 109 (1), 158-167 (2009)
Yang,L., Ann. N. Y. Acad. Sci. 1150, 263-266 (2008)
Jeffrey,K.D., Proc. Natl. Acad. Sci. U.S.A. 105 (24), 8452-8457 (2008)
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