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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ROR1 Antibody   

ROR1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - ROR1 Antibody AP7671D
    Overlay histogram showing A549 cells stained with AP7671d(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP7671d, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - ROR1 Antibody AP7671D
    AP7671d staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - ROR1 Antibody AP7671D
    AP7671d staining ROR1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - ROR1 Antibody AP7671D
    Immunohistochemical analysis of paraffin-embedded H. heart section using ROR1 Antibody (Cat#AP7671D). AP7671D was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - ROR1 Antibody AP7671D
    Western blot analysis of lysates from K562 cell line, human lung, mouse kidney, mouse heart tissue (from left to right), using ROR1 Antibody(Cat. #AP7671D). AP7671D was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • IHC-P - ROR1 Antibody AP7671D
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody (Cat.#AP7671d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q01973
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 104283 Da
Additional Information
Gene ID 4919
Other Names Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, ROR1, NTRKR1
Target/Specificity This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
Dilution FC~~1:25
IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsROR1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ROR1
Synonyms NTRKR1
Function Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A- mediated signaling.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm
Research Areas
Citations (0)

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Background

ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.

References

Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001).
Reddy, U.R., et al., Genomics 41(2):283-285 (1997).
Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).
Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).

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Cat# AP7671D
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(40 western blots)
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