|Application ||WB, IHC-P, E|
|Calculated MW||125090 Da|
|Antigen Region||12-42 aa|
|Other Names||Tyrosine-protein kinase receptor Tie-1, TIE1, TIE|
|Target/Specificity||This TIE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human TIE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TIE Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Specifically expressed in developing vascular endothelial cells|
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Provided below are standard protocols that you may find useful for product applications.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Verstovsek, S., et al., Cancer 94(5):1517-1521 (2002).
Tsiamis, A.C., et al., Microvasc. Res. 63(2):149-158 (2002).
Sato, T.N., et al., Nature 376(6535):70-74 (1995).
Partanen, J., et al., Mol. Cell. Biol. 12(4):1698-1707 (1992).
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