|Application ||WB, IHC-P, FC, E|
|Calculated MW||56648 Da|
|Antigen Region||445-474 aa|
|Other Names||Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, CBF-alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirus enhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, RUNX2, AML3, CBFA1, OSF2, PEBP2A|
|Target/Specificity||This RUNX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids surrounding S465 of human RUNX2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RUNX2 Antibody (S533) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AML3, CBFA1, OSF2, PEBP2A|
|Function||Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.|
|Tissue Location||Specifically expressed in osteoblasts.|
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Provided below are standard protocols that you may find useful for product applications.
Runx2 is a member of the RUNX family of transcription factors. It is a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in the Runx2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD).
Rich,J.T., Biochem. Biophys. Res. Commun. 372 (1), 230-235 (2008) Ermakov,S., Ann. Hum. Genet. 72 (PT 4), 510-518 (2008) Endo,T., J. Clin. Endocrinol. Metab. 93 (6), 2409-2412 (2008)
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