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DAXX Antibody (S517)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP7773b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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DAXX Antibody (S517) - Product info | |
| Application | IHC, WB
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| Primary Accession | Q9UER7 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 81373 Da |
DAXX Antibody (S517) - Additional info | |
| Gene ID 1616 | |
| Other Names DAXX; BING2; DAP6; Death domain-associated protein 6; Daxx; ETS1-associated protein 1; Fas death domain-associated protein | |
| Target/Specificity This DAXX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S517 of human DAXX. | |
| Dilution IHC~~1:10~50 WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions DAXX Antibody (S517) is for research use only and not for use in diagnostic or therapeutic procedures. | |
DAXX Antibody (S517) - Protein Information | |
| Name DAXX | |
| Synonyms BING2, DAP6 | |
| Function Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2 Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6- dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional corepressor and inhibits PAX3 and ETS1 through direct protein-protein interaction Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively | |
| Cellular Location Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere Note=Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress Colocalizes with RASSF1 in the nucleus. Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures | |
| Tissue Location Ubiquitous. | |
DAXX Antibody (S517) - Related products
AP3082a: Phospho-DAXX-S213 Antibody
AP3083a: Phospho-DAXX-S495 Antibody
AP3084a: Phospho-DAXX-S517 Antibody
AP3532a: Phospho-DAXX-pS671 Antibody
BP3082a: Phospho-DAXX-S213 Antibody Blocking Peptide
BP3083a: Phospho-DAXX-S495 Antibody Blocking Peptide
BP3084a: Phospho-DAXX-S517 Antibody Blocking Peptide
BP3532a: Phospho-DAXX-pS671 Antibody Blocking Peptide
DAXX Antibody (S517) - Application data
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with DAXX Antibody (S517) (Cat.#AP7773b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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DAXX Antibody (pS517) (Cat. #AP7773b) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DAXX antibody detected the DAXX protein (arrow).
DAXX Antibody (S517) - Research Areas
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BACKGROUND
DAXX is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, this protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, this protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination.
REFERENCES
Cantrell, S.R., et al., J. Virol. 79(12):7792-7802 (2005). Greger, J.G., et al., J. Virol. 79(8):4610-4618 (2005). Chang, C.C., et al., J. Biol. Chem. 280(11):10164-10173 (2005). Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004). Gostissa, M., et al., J. Biol. Chem. 279(46):48013-48023 (2004).
