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BACE1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BACE1 Antibody (N-term) AP7774b
    Anti-BACE1 Antibody (N-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - BACE1 Antibody (N-term) AP7774b
    Western blot analysis of anti-BACE1 Antibody (N-term) (Cat.#AP7774b) in mouse heart tissue lysates (35ug/lane). BACE1(arrow) was detected using the purified Pab.
  • IHC-P - BACE1 Antibody (N-term) AP7774b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with BACE1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56817
Other Accession P56819, P56818, Q2HJ40
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 55711 Da
Antigen Region 38-70 aa
Additional Information
Gene ID 23621
Other Names Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, BACE1, BACE, KIAA1149
Target/Specificity This BACE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-70 amino acids from the N-terminal region of human BACE1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBACE1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BACE1
Synonyms BACE, KIAA1149
Function Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Cellular Location Membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane Note=Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface
Tissue Location Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata
Research Areas
Citations ( 0 )


Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is BACE. This protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi.


Xie, J., et al., J. Biol. Chem. 280(14):13824-13832 (2005).
He, X., et al., J. Biol. Chem. 280(12):11696-11703 (2005).
Huang, X.P., et al., J. Biol. Chem. 279(36):37886-37894 (2004).
Chiocco, M.J., et al., J. Biol. Chem. 279(50):52535-52542 (2004).
Yang, H.C., et al., J. Neurochem. 91(6):1249-1259 (2004).

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$ 295.00
$ 99.00
Cat# AP7774b
(40 western blots)
Availability: In Stock
Bulk Size
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