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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CYP3A4 Antibody (Center)   

CYP3A4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CYP3A4 Antibody (Center) AP7788c
    Western blot analysis of lysate from human ovary tissue, using CYP3A4 Antibody (Center)(Cat. #AP7788c). AP7788c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - CYP3A4 Antibody (Center) AP7788c
    Western blot analysis of lysates from HepG2, A549, HT-29 cell line, human kidney and liver tissue lysate (from left to right), using CYP3A4 Antibody (Center)(Cat. #AP7788c). AP7788c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - CYP3A4 Antibody (Center) AP7788c
    Western blot analysis of CYP3A4 Antibody (Center) (Cat. #AP7788c) in NCI-H460 cell line lysates (35ug/lane). CYP3A4 (arrow) was detected using the purified Pab.
  • IHC-P - CYP3A4 Antibody (Center) AP7788c
    CYP3A4 Antibody (Center) (Cat. #AP7788c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP3A4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - CYP3A4 Antibody (Center) AP7788c
    CYP3A4 Antibody (Center) (Cat. #AP7788c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08684
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57343 Da
Antigen Region 228-255 aa
Additional Information
Gene ID 1576
Other Names Cytochrome P450 3A4, 11413-, 8-cineole 2-exo-monooxygenase, Albendazole monooxygenase, Albendazole sulfoxidase, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 HLp, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Nifedipine oxidase, Quinine 3-monooxygenase, Taurochenodeoxycholate 6-alpha-hydroxylase, CYP3A4, CYP3A3
Target/Specificity This CYP3A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human CYP3A4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP3A4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP3A4
Synonyms CYP3A3
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein
Tissue Location Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056) According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
Research Areas
Citations (0)

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CYP3A4, is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens.


Sandanaraj,E., Clin. Cancer Res. 14 (21), 7116-7126 (2008)
Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004)
Inoue,K., Jpn. J. Hum. Genet. 37 (2), 133-138 (1992)

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$ 295.00
$ 99.00
Cat# AP7788c
(40 western blots)
Availability: In Stock
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