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IRS2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - IRS2 Antibody (C-term) AP7789B
    Anti-IRS2 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 137 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - IRS2 Antibody (C-term) AP7789B
    Fluorescent image of Hela cell stained with IRS2 Antibody (C-term)(Cat#AP7789b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).IRS2 immunoreactivity is localized to Cytoplasm significantly.
  • WB - IRS2 Antibody (C-term) AP7789B
    Western blot analysis of anti-IRS2 Antibody (C-term) (Cat.#AP7789b) in 293 cell line lysates (35ug/lane). IRS2 (arrow) was detected using the purified Pab.
  • IHC-P - IRS2 Antibody (C-term) AP7789B
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9Y4H2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 1309-1338 aa
Additional Information
Other Names Insulin receptor substrate 2, IRS-2, IRS2
Target/Specificity This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIRS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IRS2
Function May mediate the control of various cellular processes by insulin.
Cellular Location Cytoplasm, cytosol. EMBL; AB000732; BAA24500.1; -; Genomic_DNA EMBL; AF073310; AAD21531.1; -; mRNA EMBL; AF322115; AAG50013.1; -; Genomic_DNA EMBL; AF322114; AAG50013.1; JOINED; Genomic_DNA EMBL; AL162497; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AF288517; AAK83053.1; -; Genomic_DNA CCDS; CCDS9510.1; - RefSeq; NP_003740.2; NM_003749.2 UniGene; Hs.442344; - PDB; 3FQW; X-ray; 1.93 A; C=1097-1105 PDB; 3FQX; X-ray; 1.70 A; C=1097-1105 PDBsum; 3FQW; - PDBsum; 3FQX; - ProteinModelPortal; Q9Y4H2; - SMR; Q9Y4H2; - BioGrid; 114209; 45 ELM; Q9Y4H2; - IntAct; Q9Y4H2; 18 MINT; Q9Y4H2; - STRING; 9606.ENSP00000365016; - iPTMnet; Q9Y4H2; - PhosphoSitePlus; Q9Y4H2; - BioMuta; IRS2; - DMDM; 62298062; - EPD; Q9Y4H2; - MaxQB; Q9Y4H2; - PaxDb; Q9Y4H2; - PeptideAtlas; Q9Y4H2; - PRIDE; Q9Y4H2; - ProteomicsDB; 86205; - DNASU; 8660; - Ensembl; ENST00000375856; ENSP00000365016; ENSG00000185950 GeneID; 8660; - KEGG; hsa:8660; - UCSC; uc001vqv.4; human CTD; 8660; - DisGeNET; 8660; - EuPathDB; HostDB:ENSG00000185950.8; - GeneCards; IRS2; - HGNC; HGNC:6126; IRS2 HPA; CAB016944; - HPA; HPA054664; - MalaCards; IRS2; - MIM; 600797; gene neXtProt; NX_Q9Y4H2; - OpenTargets; ENSG00000185950; - PharmGKB; PA375; - eggNOG; ENOG410IXEK; Eukaryota eggNOG; ENOG410Z9EP; LUCA GeneTree; ENSGT00530000063420; - HOVERGEN; HBG000542; - InParanoid; Q9Y4H2; - KO; K07187; - OMA; MHEYPLP; - OrthoDB; EOG091G02EF; - PhylomeDB; Q9Y4H2; - TreeFam; TF325994; - Reactome; R-HSA-109704; PI3K Cascade Reactome; R-HSA-112412; SOS-mediated signalling Reactome; R-HSA-1257604; PIP3 activates AKT signaling Reactome; R-HSA-1266695; Interleukin-7 signaling Reactome; R-HSA-198203; PI3K/AKT activation Reactome; R-HSA-2219530; Constitutive Signaling by Aberrant PI3K in Cancer Reactome; R-HSA-2428928; IRS-related events triggered by IGF1R Reactome; R-HSA-2586552; Signaling by Leptin Reactome; R-HSA-5673001; RAF/MAP kinase cascade Reactome; R-HSA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling Reactome; R-HSA-74713; IRS activation Reactome; R-HSA-74749; Signal attenuation Reactome; R-HSA-8853659; RET signaling Reactome; R-HSA-982772; Growth hormone receptor signaling SignaLink; Q9Y4H2; - SIGNOR; Q9Y4H2; - ChiTaRS; IRS2; human EvolutionaryTrace; Q9Y4H2; - GeneWiki; IRS2; - GenomeRNAi; 8660; - PMAP-CutDB; Q9Y4H2; - PRO; PR:Q9Y4H2; - Proteomes; UP000005640; Chromosome 13 Bgee; ENSG00000185950; - CleanEx; HS_IRS2; - Genevisible; Q9Y4H2; HS GO; GO:0005829; C:cytosol; IBA:GO_Central GO; GO:0005886; C:plasma membrane; IBA:GO_Central GO; GO:0032991; C:protein-containing complex; IEA:Ensembl GO; GO:0016303; F:1-phosphatidylinositol-3-kinase activity; TAS:Reactome GO; GO:0071889; F:14-3-3 protein binding; IEA:Ensembl GO; GO:0005158; F:insulin receptor binding; IBA:GO_Central GO; GO:0043548; F:phosphatidylinositol 3-kinase binding; IBA:GO_Central GO; GO:0046934; F:phosphatidylinositol-4,5-bisphosphate 3-kinase activity; TAS:Reactome GO; GO:0019904; F:protein domain specific binding; IEA:Ensembl GO; GO:0019901; F:protein kinase binding; IEA:Ensembl GO; GO:0019903; F:protein phosphatase binding; IEA:Ensembl GO; GO:0005088; F:Ras guanyl-nucleotide exchange factor activity; TAS:Reactome GO; GO:0007411; P:axon guidance; TAS:Reactome GO; GO:0007420; P:brain development; IEA:Ensembl GO; GO:0008283; P:cell proliferation; IEA:Ensembl GO; GO:0071333; P:cellular response to glucose stimulus; IEA:Ensembl GO; GO:0032869; P:cellular response to insulin stimulus; IMP:BHF-UCL GO; GO:0006006; P:glucose metabolic process; TAS:ProtInc GO; GO:0008286; P:insulin receptor signaling pathway; ISS:BHF-UCL GO; GO:0038111; P:interleukin-7-mediated signaling pathway; TAS:Reactome GO; GO:0055088; P:lipid homeostasis; TAS:BHF-UCL GO; GO:0030879; P:mammary gland development; IEA:Ensembl GO; GO:0000165; P:MAPK cascade; TAS:Reactome GO; GO:0002903; P:negative regulation of B cell apoptotic process; ISS:BHF-UCL GO; GO:0033673; P:negative regulation of kinase activity; ISS:BHF-UCL GO; GO:0010748; P:negative regulation of plasma membrane long-chain fatty acid transport; IMP:BHF-UCL GO; GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome GO; GO:0030890; P:positive regulation of B cell proliferation; ISS:BHF-UCL GO; GO:0030335; P:positive regulation of cell migration; IEA:Ensembl GO; GO:0008284; P:positive regulation of cell proliferation; NAS:BHF-UCL GO; GO:0032000; P:positive regulation of fatty acid beta-oxidation; IMP:BHF-UCL GO; GO:0046326; P:positive regulation of glucose import; IMP:BHF-UCL GO; GO:0010907; P:positive regulation of glucose metabolic process; IMP:BHF-UCL GO; GO:0045725; P:positive regulation of glycogen biosynthetic process; IMP:BHF-UCL GO; GO:0032024; P:positive regulation of insulin secretion; ISS:BHF-UCL GO; GO:0002053; P:positive regulation of mesenchymal cell proliferation; IEA:Ensembl GO; GO:0051897; P:positive regulation of protein kinase B signaling; TAS:Reactome GO; GO:0019216; P:regulation of lipid metabolic process; TAS:BHF-UCL GO; GO:0009749; P:response to glucose; ISS:BHF-UCL GO; GO:0007165; P:signal transduction; TAS:ProtInc CDD; cd01204; PTB_IRS; 1 Gene3D; 2.30.29.30; -; 2 InterPro; IPR039011; IRS InterPro; IPR002404; IRS_PTB InterPro; IPR011993; PH-like_dom_sf InterPro; IPR001849; PH_domain PANTHER; PTHR10614; PTHR10614; 1 Pfam; PF02174; IRS; 1 Pfam; PF00169; PH; 1 PRINTS; PR00628; INSULINRSI SMART; SM00233; PH; 1 SMART; SM00310; PTBI; 1 PROSITE; PS51064; IRS_PTB; 1 PROSITE; PS50003; PH_DOMAIN; 1 1: Evidence at protein level; 3D-structure; Complete proteome; Cytoplasm; Methylation; Phosphoprotein; Polymorphism; Reference proteome; Transducer CHAIN 1 1338 Insulin receptor substrate 2 /FTId=PRO_0000084239 DOMAIN 16 144 PH. {ECO:0000255|PROSITE- ProRule:PRU00145} DOMAIN 194 298 IRS-type PTB. {ECO:0000255|PROSITE- ProRule:PRU00389} MOTIF 540 543 YXXM motif 1 MOTIF 598 601 YXXM motif 2 MOTIF 653 656 YXXM motif 3 MOTIF 675 678 YXXM motif 4 MOTIF 742 745 YXXM motif 5 MOTIF 823 826 YXXM motif 6 MOTIF 1072 1075 YXXM motif 7 COMPBIAS 19 28 Poly-Asn COMPBIAS 371 380 Poly-Ala COMPBIAS 447 452 Poly-Ser COMPBIAS 460 467 Poly-Pro COMPBIAS 533 537 Poly-Gly COMPBIAS 642 645 Poly-Ser COMPBIAS 694 701 Poly-Ala COMPBIAS 944 947 Poly-Ser COMPBIAS 1031 1038 Poly-Pro COMPBIAS 1265 1278 Poly-Pro MOD_RES 306 306 Phosphoserine MOD_RES 346 346 Phosphoserine MOD_RES 350 350 Phosphothreonine MOD_RES 365 365 Phosphoserine MOD_RES 384 384 Phosphoserine MOD_RES 388 388 Phosphoserine MOD_RES 391 391 Phosphoserine MOD_RES 412 412 Omega-N-methylarginine MOD_RES 520 520 Phosphothreonine MOD_RES 523 523 Phosphoserine MOD_RES 527 527 Phosphothreonine MOD_RES 540 540 Phosphotyrosine; by INSR. MOD_RES 560 560 Phosphoserine MOD_RES 577 577 Phosphoserine MOD_RES 579 579 Phosphothreonine MOD_RES 580 580 Phosphothreonine MOD_RES 594 594 Phosphoserine MOD_RES 608 608 Phosphoserine MOD_RES 620 620 Phosphoserine MOD_RES 653 653 Phosphotyrosine; by INSR {ECO:0000250|UniProtKB:P81122} MOD_RES 675 675 Phosphotyrosine; by INSR MOD_RES 679 679 Phosphoserine MOD_RES 682 682 Phosphoserine MOD_RES 735 735 Phosphoserine MOD_RES 736 736 Phosphoserine MOD_RES 770 770 Phosphoserine MOD_RES 779 779 Phosphothreonine MOD_RES 805 805 Phosphoserine MOD_RES 828 828 Phosphoserine MOD_RES 915 915 Phosphoserine MOD_RES 919 919 Phosphotyrosine; by INSR. MOD_RES 973 973 Phosphoserine MOD_RES 978 978 Phosphotyrosine; by INSR. MOD_RES 1082 1082 Phosphothreonine MOD_RES 1100 1100 Phosphoserine MOD_RES 1159 1159 Phosphothreonine MOD_RES 1162 1162 Phosphoserine MOD_RES 1174 1174 Phosphoserine MOD_RES 1176 1176 Phosphoserine MOD_RES 1186 1186 Phosphoserine MOD_RES 1203 1203 Phosphoserine MOD_RES 1253 1253 Phosphotyrosine; by INSR. VARIANT 789 789 H -> Y (in dbSNP:rs35223808) /FTId=VAR_033992 VARIANT 879 879 G -> S (in dbSNP:rs549588978) {ECO:0000269|Ref.5} /FTId=VAR_021557 VARIANT 882 882 G -> A (in dbSNP:rs201499247) {ECO:0000269|Ref.5} /FTId=VAR_021558 VARIANT 999 999 V -> M (in dbSNP:rs35927012) /FTId=VAR_033993 VARIANT 1057 1057 G -> D (in dbSNP:rs1805097) /FTId=VAR_014857 CONFLICT 28 28 N -> NN (in Ref. 2; AAD21531) CONFLICT 39 41 KQK -> NEE (in Ref. 2; AAD21531) CONFLICT 59 59 E -> K (in Ref. 1; BAA24500) CONFLICT 81 81 K -> N (in Ref. 1; BAA24500) CONFLICT 107 107 A -> P (in Ref. 1; BAA24500) CONFLICT 171 171 L -> V (in Ref. 1; BAA24500) CONFLICT 371 371 A -> R (in Ref. 2; AAD21531) CONFLICT 379 382 GARP -> AQRL (in Ref. 2; AAD21531) CONFLICT 406 406 S -> I (in Ref. 1; BAA24500) CONFLICT 410 419 GGRGSKVALL -> RAAGTKWHCF (in Ref. 1; BAA24500). CONFLICT 424 424 A -> G (in Ref. 1; BAA24500) CONFLICT 436 438 AHS -> EHL (in Ref. 1; BAA24500) CONFLICT 453 453 G -> D (in Ref. 1; BAA24500) CONFLICT 456 456 S -> W (in Ref. 1; BAA24500) CONFLICT 468 468 P -> L (in Ref. 1; BAA24500) CONFLICT 662 662 L -> F (in Ref. 2; AAD21531) CONFLICT 704 704 S -> F (in Ref. 1; BAA24500) CONFLICT 714 714 S -> F (in Ref. 1; BAA24500) CONFLICT 848 848 S -> T (in Ref. 1; BAA24500) CONFLICT 872 872 P -> R (in Ref. 2; AAD21531) CONFLICT 875 878 GRPE -> RRS (in Ref. 2; AAD21531) CONFLICT 956 956 S -> L (in Ref. 1; BAA24500) CONFLICT 1252 1252 N -> K (in Ref. 1; BAA24500) CONFLICT 1303 1303 G -> R (in Ref. 1; BAA24500) CONFLICT 1314 1338 LPPANTYASIDFLSHHLKEATIVKE -> PAPCPTTYAQH (in Ref. 1; BAA24500). SEQUENCE 1338 AA; 137334 MW; 58E569E8BDBAF3D7 CRC64; MASPPRHGPP GPASGDGPNL NNNNNNNNHS VRKCGYLRKQ KHGHKRFFVL RGPGAGGDEA TAGGGSAPQP PRLEYYESEK KWRSKAGAPK RVIALDCCLN INKRADAKHK YLIALYTKDE YFAVAAENEQ EQEGWYRALT DLVSEGRAAA GDAPPAAAPA ASCSASLPGA LGGSAGAAGA EDSYGLVAPA TAAYREVWQV NLKPKGLGQS KNLTGVYRLC LSARTIGFVK LNCEQPSVTL QLMNIRRCGH SDSFFFIEVG RSAVTGPGEL WMQADDSVVA QNIHETILEA MKALKELFEF RPRSKSQSSG SSATHPISVP GARRHHHLVN LPPSQTGLVR RSRTDSLAAT PPAAKCSSCR VRTASEGDGG AAAGAAAAGA RPVSVAGSPL SPGPVRAPLS RSHTLSGGCG GRGSKVALLP AGGALQHSRS MSMPVAHSPP AATSPGSLSS SSGHGSGSYP PPPGPHPPLP HPLHHGPGQR PSSGSASASG SPSDPGFMSL DEYGSSPGDL RAFCSHRSNT PESIAETPPA RDGGGGGEFY GYMTMDRPLS HCGRSYRRVS GDAAQDLDRG LRKRTYSLTT PARQRPVPQP SSASLDEYTL MRATFSGSAG RLCPSCPASS PKVAYHPYPE DYGDIEIGSH RSSSSNLGAD DGYMPMTPGA ALAGSGSGSC RSDDYMPMSP ASVSAPKQIL QPRAAAAAAA AVPSAGPAGP APTSAAGRTF PASGGGYKAS SPAESSPEDS GYMRMWCGSK LSMEHADGKL LPNGDYLNVS PSDAVTTGTP PDFFSAALHP GGEPLRGVPG CCYSSLPRSY KAPYTCGGDS DQYVLMSSPV GRILEEERLE PQATPGPSQA ASAFGAGPTQ PPHPVVPSPV RPSGGRPEGF LGQRGRAVRP TRLSLEGLPS LPSMHEYPLP PEPKSPGEYI NIDFGEPGAR LSPPAPPLLA SAASSSSLLS ASSPASSLGS GTPGTSSDSR QRSPLSDYMN LDFSSPKSPK PGAPSGHPVG SLDGLLSPEA SSPYPPLPPR PSASPSSSLQ PPPPPPAPGE LYRLPPASAV ATAQGPGAAS SLSSDTGDNG DYTEMAFGVA ATPPQPIAAP PKPEAARVAS PTSGVKRLSL MEQVSGVEAF LQASQPPDPH RGAKVIRADP QGGRRRHSSE TFSSTTTVTP VSPSFAHNPK RHNSASVENV SLRKSSEGGV GVGPGGGDEP PTSPRQLQPA PPLAPQGRPW TPGQPGGLVG CPGSGGSPMR RETSAGFQNG LNYIAIDVRE EPGLPPQPQP PPPPLPQPGD KSSWGRTRSL GGLISAVGVG STGGGCGGPG PGALPPANTY ASIDFLSHHL KEATIVKE
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Background

Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

References

Hagg,D.A., Int. J. Mol. Med. 21 (6), 697-704 (2008)
Platanias,L.C., J. Biol. Chem. 271 (1), 278-282 (1996)
Sun,X.J., Nature 377 (6545), 173-177 (1995)

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$ 295.00
$ 99.00
Cat# AP7789B
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