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IRS2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - IRS2 Antibody (C-term) AP7789B
    Fluorescent image of Hela cell stained with IRS2 Antibody (C-term)(Cat#AP7789b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).IRS2 immunoreactivity is localized to Cytoplasm significantly.
    detail
  • WB - IRS2 Antibody (C-term) AP7789B
    Anti-IRS2 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 137 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - IRS2 Antibody (C-term) AP7789B
    Western blot analysis of anti-IRS2 Antibody (C-term) (Cat.#AP7789b) in 293 cell line lysates (35ug/lane). IRS2 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - IRS2 Antibody (C-term) AP7789B
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession Q9Y4H2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 137334 Da
Antigen Region 1309-1338 aa
Additional Information
Gene ID 8660
Other Names Insulin receptor substrate 2, IRS-2, IRS2
Target/Specificity This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIRS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IRS2
Function May mediate the control of various cellular processes by insulin.
Cellular Location Cytoplasm, cytosol.
Research Areas
Citations (0)
citation

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Background

Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

References

Hagg,D.A., Int. J. Mol. Med. 21 (6), 697-704 (2008)
Platanias,L.C., J. Biol. Chem. 271 (1), 278-282 (1996)
Sun,X.J., Nature 377 (6545), 173-177 (1995)

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$ 365.00
$ 140.00
Cat# AP7789B
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