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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ACTN4 Antibody (C-term)   

ACTN4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ACTN4 Antibody (C-term) AP7790b
    Western blot analysis of ACTN4 Antibody (C-term) (Cat.#AP7790b) in T47D, A2058, A375, HepG2 cell line and mouse bladder tissue lysates (35ug/lane). ACTN4 (arrow) was detected using the purified Pab.
  • IHC-P - ACTN4 Antibody (C-term) AP7790b
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ACTN4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - ACTN4 Antibody (C-term) AP7790b
    Flow cytometric analysis of MDA-231 cells using ACTN4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43707
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 104854 Da
Antigen Region 785-813 aa
Additional Information
Gene ID 81
Other Names Alpha-actinin-4, F-actin cross-linking protein, Non-muscle alpha-actinin 4, ACTN4
Target/Specificity This ACTN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 785-813 amino acids from the C-terminal region of human ACTN4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTN4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTN4 (HGNC:166)
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).
Cellular Location Nucleus. Cytoplasm. Cell junction {ECO:0000250|UniProtKB:P57780}. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC- 2, and bladder cancer KU-7).
Tissue Location Widely expressed.
Research Areas
Citations ( 0 )


Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This protein is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and is thought to be involved in metastatic processes. Mutations in the gene encoding this protein have been associated with focal and segmental glomerulosclerosis.


Kikuchi,S., Clin. Cancer Res. 14 (17), 5348-5356 (2008)
Barbolina,M.V., Lab. Invest. 88 (6), 602-614 (2008)
Kimura,M., Intern. Med. 47 (12), 1099-1106 (2008)

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$ 295.00
$ 99.00
Cat# AP7790b
(40 western blots)
Availability: In Stock
Bulk Size
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